Journal: Molecular cell

Article Title: MICU1 interacts with the D-ring of the MCU pore to control its Ca 2+ flux and sensitivity to Ru360

doi: 10.1016/j.molcel.2018.09.008

Figure Lengend Snippet: A-B) Time courses of the mitochondrial clearance of the [Ca2+]c rise upon addition of a 50 μM CaCl2 bolus (50Ca) in permeabilized Ctrl (A) and MICU1-KD hepatocytes (B), with and without 100nM RuRed. Dashed line indicates complete inhibition of the mitochondrial Ca2+ uptake.

Article Snippet: Construction of the HA-tagged MICU1 mutants MICU1-EF1-D9E and MICU1-∆EMRE were generated by site-directed mutagenesis in the cDNA sequence pcDNA-DEST40 containing the wild type human MICU1 (provided by Vamsi K. Mootha).

Techniques: Inhibition

Journal: Molecular cell

Article Title: MICU1 interacts with the D-ring of the MCU pore to control its Ca 2+ flux and sensitivity to Ru360

doi: 10.1016/j.molcel.2018.09.008

Figure Lengend Snippet: A) Alignment of MICU1, 2 and 3 showing previously identified sites in MICU1 involved in interactions with EMRE and MICU1/2, and proposing a potential MCU binding domain.

Article Snippet: Construction of the HA-tagged MICU1 mutants MICU1-EF1-D9E and MICU1-∆EMRE were generated by site-directed mutagenesis in the cDNA sequence pcDNA-DEST40 containing the wild type human MICU1 (provided by Vamsi K. Mootha).

Techniques: Binding Assay

Journal: Molecular cell

Article Title: MICU1 interacts with the D-ring of the MCU pore to control its Ca 2+ flux and sensitivity to Ru360

doi: 10.1016/j.molcel.2018.09.008

Figure Lengend Snippet: A-B) SOCE-induced [Ca2+]c (A: Fura2 after calibration in μM) and [Ca2+]m (B: mt-Cepia) time courses in WT and MICU1-KO HEKs expressing the indicated MICU1 constructs, after addition of 3mM CaCl2..

Article Snippet: Construction of the HA-tagged MICU1 mutants MICU1-EF1-D9E and MICU1-∆EMRE were generated by site-directed mutagenesis in the cDNA sequence pcDNA-DEST40 containing the wild type human MICU1 (provided by Vamsi K. Mootha).

Techniques: Expressing, Construct

Journal: Molecular cell

Article Title: MICU1 interacts with the D-ring of the MCU pore to control its Ca 2+ flux and sensitivity to Ru360

doi: 10.1016/j.molcel.2018.09.008

Figure Lengend Snippet: A) Time courses of the mitochondrial clearance of the [Ca2+]c rise upon addition of a 10 μM CaCl2 bolus (10Ca) in permeabilized WT and MICU1-KO HEKs with different MICU1 constructs, with or without RuRed (3μM or 30nM).).

Article Snippet: Construction of the HA-tagged MICU1 mutants MICU1-EF1-D9E and MICU1-∆EMRE were generated by site-directed mutagenesis in the cDNA sequence pcDNA-DEST40 containing the wild type human MICU1 (provided by Vamsi K. Mootha).

Techniques: Construct

Journal: Molecular cell

Article Title: MICU1 interacts with the D-ring of the MCU pore to control its Ca 2+ flux and sensitivity to Ru360

doi: 10.1016/j.molcel.2018.09.008

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Construction of the HA-tagged MICU1 mutants MICU1-EF1-D9E and MICU1-∆EMRE were generated by site-directed mutagenesis in the cDNA sequence pcDNA-DEST40 containing the wild type human MICU1 (provided by Vamsi K. Mootha).

Techniques: Recombinant, Protease Inhibitor, DC Protein Assay, Stable Transfection, Software

Journal: eLife

Article Title: Interplay between PML NBs and HIRA for H3.3 dynamics following type I interferon stimulus

doi: 10.7554/eLife.80156

Figure Lengend Snippet:

Article Snippet: Human BJ primary foreskin fibroblasts (ATCC, CRL-2522), human IMR90 fetal lung fibroblasts (ATCC, CCL-186), human HEK 293T embryonic kidney cells (Intercell, AG) and mouse MEFs embryonic fibroblasts Pml +/+ or Pml -/- (from Dr. Lallemand-Breitenbach, and whose cell identity was authenticated by STR profiling) were cultivated in DMEM medium (Sigma-Aldrich, D6429) containing 10% of fetal calf serum (FCS) (Sigma-Aldrich, F7524), 1% of penicillin/streptomycin (Sigma-Aldrich, P4458), at 37 °C under 5% CO2 and humid atmosphere.

Techniques: Transduction, Recombinant, Plasmid Preparation, Clone Assay, Mutagenesis, Sequencing, Quantitative RT-PCR, Concentration Assay, In Situ, Software, ChIP-sequencing